The effect of clary sage oil on staphylococci responsible for wound infections Because of its antimicrobial properties clary sage oil may be applied to treat wounds and skin infections. The results of the in vitro tests encourage to use formulations containing sage oil as the active natural antimicrobial agent. xylosus with MIC values ranging from 3.75 to 7.00 µl/ml. The clary sage oil was active against Staphylococcus aureus, S. The minimal inhibitory concentration (MIC) of the tested essential oil against staphylococci by the micro-dilution broth method was determined. The constituents of clary sage oil were assayed by GC-FID-MS analysis. A comprehensive evaluation of Staphylococcus clinical strain resistance to antibiotics was performed. To determine the antibacterial activity of clary sage oil ( Salvia sclarea L.) against Staphylococcus clinical strains which were isolated from patients with wound infections. The spreading of bacterial antibiotic resistance among clinical strains of pathogenic bacteria has made investigators to search for other active antibacterial agents which could provide a valuable complement to the existing therapies. Sienkiewicz, Monika Głowacka, Anna Poznańska-Kurowska, Katarzyna Kaszuba, Andrzej Urbaniak, Anna Kowalczyk, Edward The effect of clary sage oil on staphylococci responsible for wound infections. © 2011 Institute of Food Technologists® Therefore, we suggest that clary sage seed which includes high levels of omega-3 should be irradiated with low doses (≤ 2.5 kGy) of γ-irradiation. Additionally, higher doses of irradiation also decreased the volatile components of sage seed. The present study shows that the antioxidant activity of the clary sage seed is decreased by γ-irradiation. γ-Irradiation is widely applied in the preservation of spice quality. Clary sage seed has approximately 29% oil content and this oil contains >50% of α-linolenic acid. Clary sage is one of the most popular Salvia species in Turkey and many countries. Clary sage seed including high omega-3 can be irradiated with low doses (≤ 2.5 kGy) of γ-irradiation.
Higher doses of the irradiation decreased volatile components of sage seed. The dominant volatile compounds of control sage seed were found as β-pinene (18.81%) and limonene (15.60%). The four irradiation levels caused significant differences in fatty acid composition by affecting all fatty acids except palmitic, palmitoleic, and eicosenoic acids. The main fatty acid of the sage seed was remarkably found as α-linolenic acid. While the 7 kGy dose had a positive effect on the total phenolic content and antiradical activity of the sage seed extract, all doses have negative effects on the antioxidant activity of the sage seed. However, the moisture, oil, and ash contents of the samples were affected by irradiation. There was no significant difference in protein content. Additionally, the total phenolic contents, antiradical, antioxidant activities, and volatile compounds of the clary sage seed extract were determined. They were then analyzed for their protein, ash, oil and dry matter contents, and fatty acid composition. Yalcin, Hasan Ozturk, Ismet Tulukcu, Eray Sagdic, OsmanĬlary sage seeds ( Salvia sclarea L.) were obtained from plants cultivated, and 2.5, 4.0, 5.5, and 7.0 kGy doses of γ-irradiation were applied to the clary sage seeds. Effect of γ-irradiation on bioactivity, fatty acid compositions and volatile compounds of clary sage seed ( Salvia sclarea L.).